华蟾素配伍丝裂霉素体外抑瘤作用

目的：从体外药敏实验的角度观察华蟾素注射液配伍丝裂霉素对不同肝癌细胞抑瘤效果和对正常肝细胞的毒性反应。方法：通过四甲基偶氮唑盐比色法观察低浓度华蟾素注射液、丝裂霉素及两配伍使用时对人肝癌细胞系SMMC-721、BEL—7402细胞及正常肝细胞系L-02细胞的生长抑制作用。结果：对人肝癌细胞的生长抑制作用，华蟾素及丝裂霉素两配伍使用较两单独使用显增强；丝裂霉素对正常肝细胞具有一定生长抑制作用，而华蟾素注射液则无此作用，与单独使用丝裂霉素比较，两的配伍使用对正常肝细胞的生长抑制作用未见明显增加。结论：华蟾素和丝裂霉素配伍使用可显提高对人肝癌细胞的杀伤作用，同时未增加对正常肝细胞的生长抑制作用，提示华蟾素和丝裂霉素配伍使用在肝癌治疗中具有很好的临床应用前景。     

香丹注射液治疗心肌缺血血瘀证32例

目的：观察香丹注射液治疗心肌缺血（血瘀证）的临床疗效。方法：68例心电图提示心肌缺血且中医辨证属血瘀证患者随机分为西药对照组（简称对照组）30例和香丹注射液治疗组（简称治疗组）38例，对照组予消心痛5－10g，每日3次，或鲁南欣康10－20mg，每日2次；治疗组在对照组治疗基础上，用香丹注射液20ml加入50g/L葡萄糖溶液200ml中静滴，疗程均为14d。结果：治疗组总有效率达94．74％，对照组为76．67％，两组比较差异显著（P＜0．05）；治疗组胸闷，心绞痛，舌紫暗等症状，体征消减率均高于对照组（P＜0．05或P＜0．01）；心电图及血液流变性各项指标的改善均较对照组明显（P＜0．05或P＜0．01）。结论：香丹注射液对血瘀证的多种心脏病所致心肌缺血有较好疗效。     

“携带凶器抢夺”适用的思考——刑法第267条第2款释疑

最高人民法院《关于审理抢劫案件具体应用法律若干问题的解释》第6条对“携带凶器抢夺”的两种情形明确界定为抢劫罪。但是在司法实践中对“携带凶器抢夺”的适用存在困难和分歧;因此应正确对“携带凶器抢夺”予以定性,并正确适用其构成抢夺罪、抢劫罪、转化型抢劫罪的情形。     

CELO病毒fiber1基因部分片段的原核表达及抗原性鉴定

根据鸡胚致死孤儿(CELO)病毒Phelps株的fiber1基因(GenBank序列号U46933)序列设计了1对引物,采用PCR扩增CELO病毒fiber1基因C端(包含Knobs区)的部分片段,并将其克隆到质粒表达载体pGEX6p1,获得的阳性克隆命名为pGEXAF807。序列分析表明,所获得的DNA片段核苷酸序列与CELO病毒Phelps株的fiber1基因序列完全一致。将pGEXAF807转化大肠埃希氏菌DH5α,经37℃、0.6mmol/LIPTG诱导表达5h,SDSPAGE分析超声裂解后的上清和沉淀。结果显示,目的蛋白以包涵体形式存在,蛋白分子质量约为54.6ku,目的蛋白量占菌体总蛋白的35.7%。用CELO病毒多克隆血清做Westernblotting试验,结果表明目的蛋白具有较好的抗原活性。     

Suicide by Cop Among Officer-Involved Shooting Cases

Abstract: The frequency and characteristics of suicide by cop cases (SBC) among a large (n = 707) nonrandom sample of North American officer-involved shootings (OIS) were investigated. “Suicide by cop” is when a subject engages in behavior which poses an apparent risk of serious injury or death, with the intent to precipitate the use of deadly force by law enforcement against the subject. Thirty-six percent of the OIS in this sample were found to be SBC with high interrater agreement (intraclass correlation coefficient = 0.93) for category classification. SBC cases were more likely to result in the death or injury of the subjects than regular OIS cases. Most SBC cases were spontaneous, but had clear verbal and behavioral indicators that occurred prior to, and during the event. Findings confirm the trend detected in earlier research that there was a growing incidence of SBC among OIS. SBC individuals had a high likelihood of possessing a weapon (80%), which was a firearm 60% of the time. Half of those with a firearm discharged it at the police during the encounter. Nineteen percent simulated weapon possession to accomplish their suicidal intent. Other findings highlight the histories and commonalities in this high risk group.     

沃尼妙林注射液的研制

以含量测定为指标,通过均匀设计试验进行处方筛选,同时优化制备工艺;用高效液相色谱(HPLC)法对沃尼妙林注射液的含量进行检测;通过经典恒温加速试验,进行初步的稳定性试验研究.结果,优选得到的注射剂的工艺处方组成稳定.HPLC法的回收率为99.59%,检测限为0.030 4 μg/mL,定量限为0.085 2μg/mL,回归方程y=1E+06x-25 120,相关系数r2=0.999 7.结果表明,研发的沃尼妙林注射液较稳定,适合产业化生产.     

七种药物对微小牛蜱的半数致死浓度测定

用浸渍法测定了楝素、三氟氯氰菊酯、苦皮藤、高效氯氰菊酯、溴氰菊酯、哒嗪酮、螨净 7种药物对未吸血的微小牛蜱 (Boophilusmicroplus)幼蜱、若蜱、成蜱及其饱血雌蜱的半数致死浓度(LC50 )。结果显示 ,所用药物中半数致死浓度最低的 3种药物是楝素、三氟氯氰菊酯、苦皮藤。植物性杀虫剂楝素对未吸血微小牛蜱的幼蜱、若蜱、成蜱及饱血雌蜱的半数致死浓度分别为3.0 1～ 3.13、4 .37～ 4 .77、11.18～ 11.36、2 75 .5 0～ 2 76 .5 0mg/L ;植物性杀虫剂苦皮藤对未吸血微小牛蜱的幼蜱、若蜱、成蜱及饱血雌蜱的半数致死浓度分别为 4 .2 2～ 4 .4 2、10 .30～ 10 .5 0、82 .5 0～82 .70、6 35 .30～ 6 36 .70mg/L ;人工合成的除虫菊酯类的三氟氯氰菊酯对未吸血微小牛蜱的幼蜱、若蜱、成蜱及饱血雌蜱的半数致死浓度分别为 3.4 2～ 3.4 4、7.10～ 9.0 6、4 2 .30～ 4 2 .5 0、5 45 .5 0～5 46 .70mg/L。     

Death following injection sclerotherapy due to phenol toxicity

Prolapse rectum (PR) or protrusion of the rectum beyond the anus occurs frequently in populations at both extremes of age. In the pediatric population, in developed countries, the commonest cause for PR is thought to be cystic fibrosis (CF). Treatment options for CF include conservative management, surgical resection and fixation, suturing, and injection sclerotherapy (IS). The last is considered an attractive treatment option because it is minimally invasive. In this case report, the authors present the details about a 2-year-old female child, with PR and CF, who died after IS, using phenol as the sclerotherapeutic agent. Autopsy findings and toxicology tests performed to establish phenol toxicity are documented. The available literature is reviewed. This case report underscores the risks of using phenol for IS and emphasizes the point that the procedure is not innocuous and an adverse outcome including fatality is a possibility.     

丹参注射液对格列喹酮在糖尿病大鼠体内药物代谢动力学参数和肠吸收特性的影响

目的 考察丹参注射液对格列喹酮在糖尿病大鼠体内药物代谢动力学参数的影响，为两药的临床合用提供依据。方法 链脲佐菌素腹腔注射建立糖尿病大鼠模型，考察丹参注射液对格列喹酮药物代谢动力学参数的影响；建立原位在体肠循环模型，研究丹参注射液对格列喹酮肠吸收特性的影响。结果 糖尿病大鼠的药物代谢动力学研究结果表明，两药合用能够显著缩短格列喹酮的达峰时间，增加最大药物浓度、药时曲线下面积，并且可以显著减少清除率。肠吸收特性实验结果显示，丹参注射液可以显著增加格列喹酮在糖尿病大鼠体内吸收速率和表观渗透系数。结论 丹参注射液和格列喹酮合用可以显著提高糖尿病大鼠格列喹酮的血药浓度和生物利用度，其原因可能与丹参注射液促进格列喹酮的小肠吸收相关。     

关节腔注射、运动疗法联合中药熏洗治疗粘连期肩周炎的临床观察

目的 探讨关节腔注射、运动疗法联合中药熏洗治疗粘连期肩周炎的临床疗效。方法 将90例粘连期肩周炎患者随机分为对照组和治疗组，每组45例。对照组采取关节腔注射联合运动疗法治疗，治疗组在此基础上加用中药熏洗治疗，疗程为2周。治疗前后采用视觉模拟量表（visual analogue scale，VAS）评价肩关节疼痛程度，采用肩关节功能Melle评分量表及肩关节活动度评价肩关节活动度和功能，并观察两组临床疗效。结果 治疗后两组患者VAS评分、Melle评分均较治疗前降低（P＜0.05）；两组Melle评分差值比较，差异有统计学意义（P＜0.05）。治疗后两组患者的肩关节外展、内旋、外旋活动度均较治疗前增加（P＜0.05）；治疗组患者肩关节外展、内旋、外旋活动度升高值均显著高于对照组（P＜0.05）。两组临床疗效分布比较，差异有统计学意义（P＜0.05）。结论 两种治疗方案均能减轻肩关节疼痛，改善肩关节功能，关节腔注射、运动疗法联合中药熏洗对粘连期肩周炎的疗效优于关节腔注射联合运动疗法，能更好地改善肩关节功能和活动度。